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Protein Chemistry Lab
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  • Services
    • Amino Acid Analysis
      • Amino Acid Codes
      • AAA Assay Description
      • AAA Instrumentation
      • AAA Quantitation and Data Analysis
      • AAA Sample Preparation
      • AAA Sample Submission
    • Protein Sequence Analysis
      • Edman Experimental Procedure
    • Mass Spectrometry
      • Protein/Peptide Mass Determination
        • MS Mass Instrumentation
        • MS Mass Sample Preparation
        • Procedure for MALDI-TOF Analysis
        • Procedure for Electrospray Mass Analysis
        • Accuracy of the Analysis
      • Protein Identification-Proteomics
        • MS Protein ID Instrumentation
        • Sample Preparation and Purity
        • PMF/PSD
        • LC/MS/MS
        • Accuracy of the Analysis
        • In-gel Digestion of Proteins
    • Electrophoresis
      • SDS-Polyacrylamide Gel Electrophoresis
      • 2 Dimensional Gel Electrophoresis
      • DIGE
    • Chromatography
      • Chromatography Instrumentation
      • Normal Phases
      • Reversed Phase
  • Forms
    • Amino Acid Analysis
    • Protein Sequence Analysis
    • Protein/Pectide Mass Determination
    • Protein Identification/Proteomics
    • Electrophoresis
    • Chromatography
  • Staff
    • Gayan Nawaratna, Ph.D.
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  • Lab Instruments
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  • Protocols by pdf

MS Mass Instrumentation

The PCL has a Bruker Ultraflextreme MALDI-TOF mass spectrometer equipped with delayed extraction and reflectron capabilities which is used for routine high mass accuracy analysis of peptides (<3 kDa).  The instrument can also be operated in linear mode for protein analysis (>3 kDa). The Ultraflextreme is capable of Top Down and Bottom Up peptide mass fingerprinting. It is equipped with a 2000 hertz laser that makes it also capable of Imaging Mass Spectrometry. The lab has all relevant software for mass measurement, MASCOT searching with BioTools and FlexImaging and SciLs software for image analysis.
  • MS Mass Instrumentation
  • MALDI-TOF Procedure
  • Electrospray Procedure
  • Accuracy
  • Sample Preparation
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