Automated Edman chemistry is performed on an Applied Biosystems 492 Procise Protein Sequencer. The Procise is a complete, automated chemistry and analytical system for amino acid sequence analysis of protein and peptide samples in picomole to nanomole amounts.
The DALTSix is a modular electrophoresis system for large format 2D gel electrophoresis. It can run up to 6 lab-cast or pre-cast gels (26 x 20 cm) simultaneously. It is used with 18 and 24 cm IPG DryStrips.
Typhoon Trio Fluorescent Imager
The FLA 9500 Typhoon (GE Healthcare) is a high performance gel and blot imager. The imager has powerful laser excitation sources and high-quality confocal optics that allow for the sensitive detection of low-abundance targets. Red, Green and Blue excitation wavelengths (488 nm, 532 nm, 633 nm) and a wide choice of emission filters enable imaging of an extensive variety of fluorophores. The 9500 is also useful for Storage Phosphor Autoradiography, Chemiluminescence and Chemifluorescence detection. (Client must have their own Phosphor screens. The PCL does not own any.) Please call ahead with specific questions. The instrument is used on a first-come, first-served basis, but reservations are recommended for analyses that will require extensive periods of time.
Ettan Robotic Systems
Picker automatically picks selected protein spots from stained and unstained gels. It is designed for backed as well as unbacked gels. This instrument is seamless with our DeCyder 2-D Differential Analysis software.The Picker accurately picks protein spots from 2D gels and transfers them into 96 well microplates for further treatment.
The Digester is a highly versatile instrument designed to perform in-gel digestion of proteins captured in 2D gel electrophoresis spots. Default digestion protocols can be customized. The instrument performs automatic tracking of samples.
The Ettan Spotter is designed to automatically mix and spot digested proteins and matrix onto any MALDI sample plate.
High Pressure Liquid Chromatography
The PCL has a Shimadzu/Kratos MALDI-TOF mass spectrometer equipped with delayed extraction and reflectron capabilities which is used for routine high mass accuracy analysis of peptides (<3 kDa). The instrument can also be operated in linear mode for protein analysis (>3 kDa).
The PCL has recently installed a Bruker Ultraflextreme MALDI-TOF-TOF mass spectrometer. The instrument operates in linear and reflector modes and is capable of Top Down and Bottom Up protein sequencing. In addition, with attachments that we have, the Ultraflextreme is capable of Imaging Mass Spectrometry.
Electrospray Mass Spectrometer
We are equipped with a ThermoFisher Exactive Orbitrap mass spectrometer that is used for peptide mass measurements. Samples can be applied by direct infusion or after in-line reversed phase chromatographic separation.