Instrumentation
Automated Edman chemistry is performed on an Applied Biosystems 492 Procise Protein Sequencer. The Procise is a complete, automated chemistry and analytical system for amino acid sequence analysis of protein and peptide samples in picomole to nanomole amounts.
The Procise 492 Protein Sequencer performs automated Edman sequencing chemistry on a Biobrene-coated Glass Fiber Filter (for liquid samples) or on proteins blotted to PVDF after PAGE separation. Sensitivity is in the low pmol range.