Mass spectrometry imaging (MSI) is a powerful analytical technique that allows for the spatial localization of chemical compounds within biological tissues and other complex samples. By combining mass spectrometry with imaging technologies, MSI provides detailed molecular maps, revealing the distribution of metabolites, proteins, and lipids at high spatial resolution. This innovative approach is transforming fields such as proteomics, pharmacology, and cancer research, enabling scientists to visualize biomolecular distributions in ways that were previously unattainable.