Circular Dichroism Spectroscopy
Circular Dichroism (CD) spectrophotometer enables the collection of spectra and single-wavelength data within both the far UV (195–260 nm, peptide bond region) and near UV (260–320 nm, aromatic region) ranges.
The CD spectrophotometer is equipped with a software-controlled magnetic stirrer and a Peltier cell holder, facilitating temperature-controlled measurements from 0 to 100 degrees Celsius. The cell holder accommodates cuvettes with pathlengths of 10, 5, 2, 1, and sub-1 mm, allowing for flexibility in experimental design.
It is critical to select the appropriate cell pathlength and sample buffer composition to ensure the acquisition of high-quality data. It is recommended to utilize ultra-pure chemicals and to avoid chloride ions, carbon-based buffers (such as HEPES, MOPS, citrate, and imidazole) and reducing agents like dithiothreitol (DTT) to minimize interference with CD measurements.
